The enzymic phosphorylation of vitamin B1

Non-enzymic protein phosphorylation

Incubation of 6-phosphogluconate dehydrogenase from Candida utilis with either acetyl phosphate, 1,3-diphosphoglycerate or carbamoyl phosphate results in the phosphorylation of the protein. The binding of one phosphate residue per enzyme subunit does not affect significantly the kinetic properties, but makes the enzyme less reactive toward thiol reagents, trypsin and pyridoxal 5'-phosphate. We ...

Vitamin B1

Vitamin B1 is a watersoluble vitamin. It consists out of a pyrimidineand a thiazol ring, which are synthesized independently and linked afterwards.Vitamin B1 is de novo produced by many plants and microorganisms. Humans and animals have to take it up by nutrition. They are only able to do the phosphorylation reaction of the thiamin molecule. Thiamin is found free and as mono-, diand triphosphat...

Vitamin-B1 Deficiency

Xci . the Reversible Enzymic Oxidation of Vitamin C

SOME years ago during the progress of an inquiry on the production of an antiscorbutically active cider from Bramley's Seedling apples it was observed that the juice expressed from the fruit while retaining a very high proportion of its antiscorbutic activity did not reduce indophenol. At that time the writer held the view that the capacity for reducing this indicator was mainly due to a substa...

Intestinal Flora and Vitamin B1